Western blot using the affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000.

Polyclonal Rabbit anti-Mouse EIF3E Antibody (C-Terminus, WB)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:	Mouse
Format:	Liquid

$311.00each

Catalog Number: LS-C745313-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: EIF3E antibody LS-C745313 is an unconjugated rabbit polyclonal antibody to EIF3E (C-Terminus) from mouse. It is reactive with human, mouse, rat and other species. Validated for ELISA and WB.
Application: ELISA, WB
Reactivity: Ms
Predicted Reactivity: Bov, Can, Hu, NHP, Rt
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: EIF3-p46 | EIF3E | INT6 | EIF-3 p48 | EIF3-P48 | EIF3S6
Recommended Dilution: ELISA: 1:80000
Recommended Dilution: WB: 1:1000
UniProt Number: P60228
NCBI GenBank: NM_001568, NP_001559.1
SwissProt: P60228

Target

Target: Mouse EIF3E
Antigen Species: Ms
Epitope: This antibody is specific for mouse eIF3S6/Int6 protein. A BLAST analysis was used to suggest cross-reactivity with most eIF3Se/Int6 isoforms from mouse, human, rat, dog, bovine, and monkey based on 100% homology with the immunizing sequence. Cross-reactivity with EIF3S6/Int6 from other sources has not been determined.
Gene Name: EIF3E

Antibody

Host Species: Rb
Clonality: Polyclonal
Isotype: IgG
Origin Species: Hu

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-Terminus of mouse EIF3S6/Int6.
Immunogen Type: SP-Lin

Format

Conjugate/Label: Unconj
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 0.8 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: AP

Storage & Handling

Storage Conditions: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl