LSBio
Polyclonal Rabbit anti-Mouse EIF3E Antibody (C-Terminus, WB)

Anti-eIF3S6/Int6 Antibody - Western Blot. Western blot of affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kD, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000. Personal communication, J. Lee, NCI, Bethesda, MD.

Polyclonal Rabbit anti-Mouse EIF3E Antibody (C-Terminus, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Mouse
Format:Liquid
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$579.00each
Catalog Number: LS-C154233-100

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Overview

Description
EIF3E antibody LS-C154233 is an unconjugated rabbit polyclonal antibody to EIF3E (C-Terminus) from mouse. It is reactive with human, mouse, rat and other species. Validated for ELISA and WB.
Application
ELISA, IHC, WB
Reactivity
Ms
Predicted Reactivity
Bovine, Dog, Human, Non-Human Primate, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
EIF3-p46 | EIF3E | INT6 | EIF-3 p48 | EIF3-P48 | EIF3S6
Recommended Dilution: ELISA
1:90000
Recommended Dilution: WB
1:1000
UniProt Number
P60228
SwissProt
P60228

Target

Target
Mouse EIF3E
Antigen Species
Mouse
Epitope
This antibody is specific for mouse eIF3S6/Int6 protein. A BLAST analysis was used to suggest cross-reactivity with most eIF3Se/Int6 isoforms from mouse, human, rat, dog, bovine, and monkey based on 100% homology with the immunizing sequence. Cross-reactivity with EIF3S6/Int6 from other sources has not been determined.
Gene Name
EIF3E

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-Terminus of mouse EIF3S6/Int6.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.8 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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