Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western blot using the affinity purified anti-ATDC (Ac-K116) antibody shows detection of a 66 kDa band corresponding to over-expressed, acetylated lysine (K116) ATDC (arrowhead) in transfected 293T cells. No staining is noted for cells transfected with empty vector only. No staining is noted for cells transfected with an ATDC K116R mutant (K to R transversion lacks site for acetylation). In each instance, samples were prepared with and without TSA (1.3uM, 6 hr) which inhibits deacetylation.
Polyclonal Rabbit anti-Human TRIM29 Antibody (Lys116, Internal, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Cynomolgus Monkey, Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745328-25
Catalog Number: LS-C745328
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- Description
- TRIM29 antibody LS-C745328 is an unconjugated rabbit polyclonal antibody to TRIM29 (Lys116, Internal) from human. It is reactive with human, bovine, chimpanzee and other species. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Cyno, Hu
- Predicted Reactivity
- Bov, Cyno, Hr, Ms, NHP, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ATDC | Tripartite motif containing 29 | TRIM29 | Tripartite motif-containing 29
- Recommended Dilution: ELISA
- 1:100000
- Recommended Dilution: WB
- 1:500 - 1:3000
- UniProt Number
- Q14134
- NCBI GenBank
- NM_012101, NP_036233.2
- SwissProt
- Q14134
Target
- Target
- Human TRIM29
- Antigen Species
- Hu
- Epitope
- This antibody reacts with over-expressed, acetylated lysine ATDC protein. A BLAST analysis was used to suggest cross-reactivity with ATDC from human, horse, cattle, chimpanzee and macaque based on a 100% homology with the immunizing sequence. Partial reactivity is expected against rat and mouse ATDC based on 92% homology with the immunizing sequence. Cross-reactivity with ATDC from other sources has not been determined.
- Gene Name
- TRIM29
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human ATDC protein around lysine 116.
- Immunogen Type
- Pep
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.67 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
