Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Anti-ATDC (Ac-K116) Antibody - Western Blot. Western blot of affinity purified anti-ATDC (Ac-K116) antibody shows detection of a 66 kD band corresponding to over-expressed, acetylated lysine (K116) ATDC (arrowhead) in transfected 293T cells. No staining is noted for cells transfected with empty vector only. No staining is noted for cells transfected with an ATDC K116R mutant (K to R transversion lacks site for acetylation). In each instance, samples were prepared with and without TSA (1.3uM, 6 hr) which inhibits deacetylation. Personal communication, Z. Yuan, H Lee Moffitt Cancer Center and Research Institute.
TRIM29 Antibody (Lys116, Internal)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Cynomolgus Monkey, Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C154244-100
Catalog Number: LS-C154244
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- TRIM29 antibody LS-C154244 is an unconjugated rabbit polyclonal antibody to TRIM29 (Lys116, Internal) from human. It is reactive with human, bovine, chimpanzee and other species. Validated for ELISA and WB. Cited in 2 publications.
- Application
- ELISA, IHC, WB
- Reactivity
- Cyno, Hu
- Predicted Reactivity
- Bovine, Cynomolgus Monkey, Horse, Mouse, Non-Human Primate, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ATDC | Tripartite motif containing 29 | TRIM29 | Tripartite motif-containing 29
- Recommended Dilution: ELISA
- 1:100000
- Recommended Dilution: WB
- 1:500 - 1:3000
- UniProt Number
- Q14134
- NCBI GenBank
- NM_012101 | NP_036233.2
- SwissProt
- Q14134
Target
- Target
- Human TRIM29
- Antigen Species
- Human
- Epitope
- This antibody reacts with over-expressed, acetylated lysine ATDC protein. A BLAST analysis was used to suggest cross-reactivity with ATDC from human, horse, cattle, chimpanzee and macaque based on a 100% homology with the immunizing sequence. Partial reactivity is expected against rat and mouse ATDC based on 92% homology with the immunizing sequence. Cross-reactivity with ATDC from other sources has not been determined.
- Gene Name
- TRIM29
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human ATDC protein around lysine 116.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.67 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
The ATDC (TRIM29) protein binds p53 and antagonizes p53-mediated functions. Yuan Z, Villagra A, Peng L, Coppola D, Glozak M, Sotomayor EM, Chen J, Lane WS, Seto E. Molecular and cellular biology. 2010;30:3004-15. PubMed: 20368352 PMC: PMC2876676
