Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Fluorescent image of U251 cells stained with SQSTM1 (p62) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SQSTM1 (p62) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). SQSTM1 (p62) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000161011).
Polyclonal Rabbit anti-Human SQSTM1 Antibody (aa317-346, IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C161484-400
Catalog Number: LS-C161484
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- SQSTM1 antibody LS-C161484 is an unconjugated rabbit polyclonal antibody to human SQSTM1 (aa317-346). Validated for IF, IHC and WB. Cited in 2 publications.
- Application
- IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- A170 | ORCA | OSIL | Paget disease of bone 3 | p60 | p62 | PDB3 | Sequestosome-1 | Ubiquitin-binding protein p62 | Oxidative stress induced like | p62B | ZIP3 | Sequestosome 1 | SQSTM1 | EBI3-associated protein p60 | EBIAP
- Recommended Dilution: IF/ICC
- 1:50 - 1:100
- Recommended Dilution: IHC-P
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q13501
- NCBI GenBank
- NM_003900 | NP_003891.1
- SwissProt
- Q13501
Target
- Target
- Human SQSTM1
- Antigen Species
- Human
- Epitope
- This SQSTM1 (p62) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human SQSTM1 (p62).
- Gene Name
- SQSTM1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS
Impaired protein aggregate handling and clearance underlie the pathogenesis of p97/VCP-associated disease. Ju JS, Miller SE, Hanson PI, Weihl CC. The Journal of biological chemistry. 2008;283:30289-99.
PubMed: 18715868 · PMC: PMC2573070
Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease. Nascimento-Ferreira I, Santos-Ferreira T, Sousa-Ferreira L, Auregan G, Onofre I, Alves S, Dufour N, Colomer Gould VF, Koeppen A, Dglon N, Pereira de Almeida L. Brain : a journal of neurology. 2011;134:1400-15.
PubMed: 21478185
