Western Blot of rabbit anti-POLB antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: Raji Lsyate Lane 7: Ramos Lysate Lane 8: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: POLB antibody at 1:5,000 for 3hrs at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 38 kDa for POLB.
POLB / DNA Polymerase Beta Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745350-25
Catalog Number: LS-C745350
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- Description
- DNA Polymerase Beta antibody LS-C745350 is an unconjugated rabbit polyclonal antibody to human DNA Polymerase Beta (POLB) (C-Terminus). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Xenopus
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DNA pol beta | Mutant DNA polymerase beta | POLB | DNA polymerase beta | DNA polymerase beta subunit
- Recommended Dilution: ELISA
- 1:50000
- Recommended Dilution: WB
- 0.5 µg/ml
- UniProt Number
- P06746
- NCBI GenBank
- NM_002690 | NP_002681.1
- SwissProt
- P06746
Target
- Target
- Human POLB / DNA Polymerase Beta
- Antigen Species
- Human
- Epitope
- This antibody reacts with overexpressed and endogenous POLβ protein. A BLAST analysis was used to suggest reactivity with POLβ from human, bovine, and Xenopus laevis based on a 100% homology with the immunizing sequence. Cross-reactivity with POLβ from other sources has not been determined.
- Gene Name
- POLB
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POLβ protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.4 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl