LSBio
Polyclonal Rabbit anti-Human PCNA Antibody (aa75-125, IHC, WB)

Flow Cytometric Detection of PCNA v.DNA Content. Asynchronous Jurkat cells were fixed and permeabilized in a sequential treatment of FACS buffer (PBS, 0.5% triton-X-100, 0.5mM EDTA, 1% BSA) and 100% methanol. One million cells were stained with 0.03 ug anti-PCNA []. Secondary detection was performed with FITC conjugated Goat F(ab')2 anti-rabbit antibody [A120-114F], and DNA stained with PI.

Polyclonal Rabbit anti-Human PCNA Antibody (aa75-125, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human PCNA antibodies →
$301.00each
Catalog Number: LS-C287181-10

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Overview

Description
PCNA antibody LS-C287181 is an unconjugated rabbit polyclonal antibody to PCNA (aa75-125) from human. It is reactive with human and mouse. Validated for Flow, IHC, IP and WB.
Application
FC, IHC, IP, WB
Reactivity
Hu, Ms
Predicted Reactivity
Bovine, Chicken, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Cyclin | PCNA
Recommended Dilution: IHC
1:2000 - 1:10000
Recommended Dilution: WB
1:1000 - 1:10000
UniProt Number
P12004
SwissProt
P12004

Target

Target
Human PCNA
Antigen Species
Human
Epitope
Region between residues 75 and 125 of human proliferating cell nuclear antigen using the numbering given in SwissProt entry P12004 (GeneID 5111).
Gene Name
PCNA

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
A portion of GeneID: 5111 corresponding to amino acids between 75 and 125 of UniprotID: P12004
Immunogen Type
Recombinant Protein Fragment

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 2-8°C for up to one year.
Recommended Storage Buffer
Tris-citrate

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