LSBio
PathPlus™ PCNA Antibody (clone PC10)

Anti-PCNA / Cyclin antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PathPlus™ PCNA Antibody (clone PC10)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Dog, Chicken, Cynomolgus Monkey, Cat, Fish (Other), Hamster, Horse, Human, Mammal (Other), Mouse, Non-Human Primate, Rabbit, Rat, Sheep, Xenopus, Rhesus Macaque
Format:Liquid
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$470.00each
Catalog Number: LS-B9987-0.05

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Overview

Description
PCNA antibody LS-B9987 is an unconjugated mouse monoclonal antibody to PCNA from rat. It is reactive with human, mouse, rat and other species. Validated for Flow, IHC, IP and WB.
Application
FC, IHC, IHC-F, IHC-P, IP, WB
Reactivity
Can, Ck, Cyno, Fel, Fish, Ham, Hr, Hu, Mml, Ms, NHP, Rb, Rt, Sh, Xen, Rhes
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Cyclin | PCNA
Recommended Dilution: FC
1:100
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:1000
UniProt Number
P12004
SwissProt
P12004

Target

Target
PCNA
Antigen Species
Rat
Specificity
Recognizes the proliferating cell nuclear antigen (PCNA), a nuclear protein vital for cellular DNA synthesis
Epitope
Recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kD nuclear protein vital for cellular DNA synthesis at the replication fork through its interaction with. PCNA is the auxilliary protein for DNA polymerase delta. PCNA is highly conserved between mammalian species and other vertebrates. Clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse, chicken and abalone.
Gene Name
PCNA

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
PC10
Isotype
IgG2a
Origin Species
Human

Immunogen

Immunogen
Rat PCNA made in the protein A expression vector pR1T2T.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Short term: store at 4°C for up to 2 weeks. Long term: aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.