LSBio
Polyclonal Rabbit anti-Human MAP1LC3B / LC3B Antibody (aa1-30, IHC, IF, WB)

Western blot of lysates from HepG2 cell line, untreated or treated with chloroquine, using LC3 Antibody (upper) or Beta-actin (lower).

Polyclonal Rabbit anti-Human MAP1LC3B / LC3B Antibody (aa1-30, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Rat
Format:Liquid
See all Human MAP1LC3B / LC3B antibodies →
$401.00each
Catalog Number: LS-C165711-200

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Overview

Description
LC3B antibody LS-C165711 is an unconjugated rabbit polyclonal antibody to LC3B (MAP1LC3B) (aa1-30) from human. It is reactive with human and rat. Validated for IF, IHC and WB. Cited in 6 publications.
Application
IF, IHC, IHC-P, WB
Reactivity
Hu, Rt
Predicted Reactivity
Bovine
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ATG8F | LC3B | MAP1LC3B | MAP1A/1BLC3 | MAP1A/MAP1B LC3 B | MAP1A/MAP1B light chain 3 B | MAP1ALC3 | MAP1LC3B-a
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: IHC-P
1:50 - 1:100
Recommended Dilution: WB
1:1000
UniProt Number
Q9GZQ8
SwissProt
Q9GZQ8

Target

Target
Human MAP1LC3B / LC3B
Antigen Species
Human
Epitope
This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.
Gene Name
MAP1LC3B

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen Type
Peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
0.4 mg/mL
Volume
200 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer
PBS

Active ras triggers death in glioblastoma cells through hyperstimulation of macropinocytosis. Overmeyer JH, Kaul A, Johnson EE, Maltese WA. Molecular cancer research : MCR. 2008;6:965-77.

PubMed: 18567800 · PMC: PMC2994605


Lysosomal degradation of endocytosed proteins depends on the chloride transport protein ClC-7. Wartosch L, Fuhrmann JC, Schweizer M, Stauber T, Jentsch TJ. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2009;23:4056-68.

PubMed: 19661288


Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C virus NS4B-induced autophagy. Su WC, Chao TC, Huang YL, Weng SC, Jeng KS, Lai MM. Journal of virology. 2011;85:10561-71.

PubMed: 21835792 · PMC: PMC3187495


Caspase-6 activity in a BACHD mouse modulates steady-state levels of mutant huntingtin protein but is not necessary for production of a 586 amino acid proteolytic fragment. Gafni J, Papanikolaou T, Degiacomo F, Holcomb J, Chen S, Menalled L, Kudwa A, Fitzpatrick J, Miller S, Ramboz S, Tuunanen PI, Lehtimki KK, Yang XW, Park L, Kwak S, Howland D, Park H, Ellerby LM. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2012;32:7454-65.

PubMed: 22649225 · PMC: PMC3454486


Chronic autophagy is a cellular adaptation to tumor acidic pH microenvironments. Wojtkowiak JW, Rothberg JM, Kumar V, Schramm KJ, Haller E, Proemsey JB, Lloyd MC, Sloane BF, Gillies RJ. Cancer research. 2012;72:3938-47.

PubMed: 22719070


Induction of autophagy by Imatinib sequesters Bcr-Abl in autophagosomes and down-regulates Bcr-Abl protein. Gafni J, Papanikolaou T, Degiacomo F, Holcomb J, Chen S, Menalled L, Kudwa A, Fitzpatrick J, Miller S, Ramboz S, Tuunanen PI, Lehtimki KK, Yang XW, Park L, Kwak S, Howland D, Park H, Ellerby LM. American journal of hematology. 2013.

PubMed: 23440701

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