Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3B / LC3B Antibody (aa89-122)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general) |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C168823-400
Catalog Number: LS-C168823
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- Description
- LC3B antibody LS-C168823 is an unconjugated rabbit polyclonal antibody to LC3B (MAP1LC3B) (aa89-122) from human. It is reactive with human and mouse. Validated for ICC, IF and IHC.
- Application
- ICC, IF, IHC
- Reactivity
- Hu, Ms
- Predicted Reactivity
- Bov
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ATG8F | LC3B | MAP1LC3B | MAP1A/1BLC3 | MAP1A/MAP1B LC3 B | MAP1A/MAP1B light chain 3 B | MAP1ALC3 | MAP1LC3B-a
- Recommended Dilution: IF/ICC
- 1:10 - 1:50
- UniProt Number
- Q9GZQ8
- NCBI GenBank
- NM_022818, NP_073729.1
- SwissProt
- Q9GZQ8
Target
- Target
- Human MAP1LC3B / LC3B
- Antigen Species
- Hu
- Epitope
- This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B.
- Gene Name
- MAP1LC3B
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Pep
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Prot A
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS