LSBio
Polyclonal Rabbit anti-Human IL7R / CD127 Antibody (WB)

Western blot analysis of extracts of various cell lines, using IL7R antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.

Polyclonal Rabbit anti-Human IL7R / CD127 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunoprecipitation, Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human IL7R / CD127 antibodies →
$281.00each
Catalog Number: LS-C748550-20

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Overview

Description
CD127 antibody LS-C748550 is an unconjugated rabbit polyclonal antibody to CD127 (IL7R) from human. It is reactive with human and mouse. Validated for IP and WB.
Application
IP, WB
Reactivity
Hu, Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CD127 | CD127 antigen | IL-7RA | IL-7 receptor subunit alpha | IL7RA | ILRA | Interleukin 7 receptor | CDW127 | IL-7R subunit alpha | IL-7R-alpha | IL7R
Recommended Dilution: IP
1:50 - 1:100
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P16871
NCBI GenBank
NM_002185 | NP_002176.2
SwissProt
P16871

Target

Target
Human IL7R / CD127
Antigen Species
Human
Gene Name
IL7R

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 21-239 of human IL7R (NP_002176.2). ESGYAQNGDLEDAELDDYSFSCYSQLEVNGSQHSLTCAFEDPDVNITNLEFEICGALVEVKCLNFRKLQEIYFIETKKFLLIGKSNICVKVGEKSLTCKKIDLTTIVKPEAPFDLSVVYREGANDFVVTFNTSHLQKKYVKVLMHDVAYRQEKDENKWTHVNLSSTKLTLLQRKLQPAAMYEIKVRSIPDHYFKGFWSEWSPSYYFRTPEINNSSGEMD
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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