LSBio
Polyclonal Rabbit anti-Human IL-33 Antibody (HRP, IHC, WB)

Anti-Human IL-33 Antibody - Western Blot. Western blot of HRP conjugated anti-Human IL-33 antibody shows detection of a band ~18 kD in size corresponding to recombinant human IL-33. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5000 dilution for 30 min at room temperature. Detection occurred using TMB substrate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Rabbit anti-Human IL-33 Antibody (HRP, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Lyophilized
See all Human IL-33 antibodies →
$564.00each
Catalog Number: LS-C154104-100

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Overview

Description
IL-33 antibody LS-C154104 is an HRP-conjugated rabbit polyclonal antibody to human IL-33. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
C9orf26 | DVS27 | DVS27-related protein | IL-1F11 | IL-33 | Interleukin-33 | NFEHEV | NF-HEV | IL33 | Interleukin-1 family member 11 | NFHEV | IL1F11 | Interleukin 33 | RP11-575C20.2
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:1000 - 1:5000
UniProt Number
O95760
SwissProt
O95760

Target

Target
Human IL-33
Antigen Species
Human
Epitope
This antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Gene Name
IL33

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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