Western blot using anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recom-binant human IL-33 (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc® MP 4000 imaging system (Bio-Rad).

IL-33 Antibody (Biotin)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Lyophilized

$564.00each

Catalog Number: LS-C154115-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: IL-33 antibody LS-C154115 is a biotin-conjugated rabbit polyclonal antibody to human IL-33. Validated for ELISA, IHC and WB.
Application: ELISA, IHC, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: C9orf26 | DVS27 | DVS27-related protein | IL-1F11 | IL-33 | Interleukin-33 | NFEHEV | NF-HEV | IL33 | Interleukin-1 family member 11 | NFHEV | IL1F11 | Interleukin 33 | RP11-575C20.2
Recommended Dilution: ELISA: 1:20000 - 1:100000
Recommended Dilution: IHC: 1:1000 - 1:5000
Recommended Dilution: WB: 1:2000 - 1:10000
UniProt Number: O95760
NCBI GenBank: NM_033439 | NP_254274.1
SwissProt: O95760

Target

Target: Human IL-33
Antigen Species: Human
Epitope: This antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Gene Name: IL33

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.
Immunogen Type: Full-Length Recombinant Protein

Format

Conjugate/Label: Biotin
Format: Lyophilized
Formulation: Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions: Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl