LSBio
Polyclonal Rabbit anti-Human BCL2 / Bcl-2 Antibody (aa41-54, IHC, WB)

Western blot of Bcl-2 using Polyclonal Antibody to Bcl-2 at 1:2000.20 ug/protein was loaded per lane. Lane 1. Human Jurkat T cells. Lane 2. Human RS11 lymphoma cells. Lanes 3-6. Human breast cancer cases. Patient sample in lane 5 lacked detectible Bcl-2 expression.

Polyclonal Rabbit anti-Human BCL2 / Bcl-2 Antibody (aa41-54, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Dog, Human, Non-Human Primate, Pig
Format:Liquid
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$394.00each
Catalog Number: LS-C148227-50

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Overview

Description
Bcl-2 antibody LS-C148227 is an unconjugated rabbit polyclonal antibody to Bcl-2 (BCL2) (aa41-54) from human. It is reactive with human, dog, monkey and other species. Validated for IHC, IP and WB.
Application
IHC, IHC-F, IHC-P, IP, WB
Reactivity
Can, Hu, NHP, Por
Predicted Reactivity
Bovine
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Apoptosis regulator Bcl-2 | BCL2 | B-cell CLL/lymphoma 2 | B-cell lymphoma protein 2 | Bcl-2 | PPP1R50
Recommended Dilution: IHC-P
1:1000 - 1:5000
Recommended Dilution: IP
1:50 - 1:200
Recommended Dilution: WB
1:1000 - 1:2000
UniProt Number
P10415
SwissProt
P10415

Target

Target
Human BCL2 / Bcl-2
Antigen Species
Human
Epitope
Bcl-2.
Gene Name
BCL2

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG) of human Bcl-2. This sequence is 100% conserved in human Bcl-2 alpha (239 aa) and beta (205aa) isoforms. This sequence is100% conserved in dog and cat Bcl-2, 92% conserved in cow Bcl-2 and 84% conserved in mouse and rat Bcl-2. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Dog, Cat, Pig (100%); Orangutan, Bovine (93%); Galago, Horse (86%); Mouse (79%); Rat (71%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Antiserum, 0.05% sodium azide
Volume
50 Microliter
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Store at -20°C. Aliquot to avoid freeze/thaw cycles.

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