LSBio
IHC-plus™ BCL2 / Bcl-2 Antibody (aa46-95)

Anti-BCL2 / Bcl-2 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

IHC-plus™ BCL2 / Bcl-2 Antibody (aa46-95)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B6548-50

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Overview

Description
Bcl-2 antibody LS-B6548 is an unconjugated rabbit polyclonal antibody to Bcl-2 (BCL2) (aa46-95) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Apoptosis regulator Bcl-2 | BCL2 | B-cell CLL/lymphoma 2 | B-cell lymphoma protein 2 | Bcl-2 | PPP1R50
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P10415
NCBI GenBank
NM_000633 | NP_000624.2
SwissProt
P10415

Target

Target
BCL2 / Bcl-2
Antigen Species
Human
Specificity
BCL-2 (Ab-69) Antibody detects endogenous levels of total BCL-2 protein.
Epitope
BCL-2 (Ab-69) Antibody detects endogenous levels of total BCL-2 protein.
Gene Name
BCL2

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from human BCL-2. (AA Range: 46-95)
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS, 150 mM NaCl
Bmi-1 in gallbladder carcinoma: Clinicopathology and mechanism of regulation of human gallbladder carcinoma proliferation. Jiao K, Jiang W, Zhao C, Su D, Zhang H. Oncology letters. 2019 August;18:1365-1371. PubMed: 31423199 PMC: PMC6607302
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