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Polyclonal Rabbit anti-Human ATP2A1 / SERCA1 Antibody (WB)

Western blot analysis of extracts of HL-60 cells, using ATP2A1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Polyclonal Rabbit anti-Human ATP2A1 / SERCA1 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Western Blot
Reactivity:Human
Format:Liquid
See all Human ATP2A1 / SERCA1 antibodies →
$281.00each
Catalog Number: LS-C496815-20

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Overview

Description
SERCA1 antibody LS-C496815 is an unconjugated rabbit polyclonal antibody to human SERCA1 (ATP2A1). Validated for WB.
Application
WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ATP2A1 | SERCA1 | ATP2A | Calcium pump 1 | SR Ca(2+)-ATPase 1
Recommended Dilution: WB
1:200 - 1:1000
UniProt Number
O14983
NCBI GenBank
NM_004320 | NP_004311.1
SwissProt
O14983

Target

Target
Human ATP2A1 / SERCA1
Antigen Species
Human
Gene Name
ATP2A1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 350-600 of human ATP2A1 (NP_775293.1). SDKTGTLTTNQMSVCKMFIIDKVDGDICLLNEFSITGSTYAPEGEVLKNDKPVRPGQYDGLVELATICALCNDSSLDFNEAKGVYEKVGEATETALTTLVEKMNVFNTDVRSLSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSSRAAVGNKMFVKGAPEGVIDRCNYVRVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVLDDSARFLEYETDLTFVGVVGML
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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