LSBio
Polyclonal Rabbit anti-Human Alpha Tubulin Antibody (Biotin, aa425-451, IHC, IF, WB)

Polyclonal Rabbit anti-Human Alpha Tubulin Antibody (Biotin, aa425-451, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Bovine, Chicken, Goat, Human, Mouse, Rat, Sheep
Format:Lyophilized
See all Human Alpha Tubulin antibodies →
$482.00each
Catalog Number: LS-C154296-100

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Overview

Description
Alpha Tubulin antibody LS-C154296 is a biotin-conjugated rabbit polyclonal antibody to Alpha Tubulin (aa425-451) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Application
ELISA, IF, IHC, WB
Reactivity
Bov, Ck, Gt, Hu, Ms, Rt, Sh
Predicted Reactivity
Bacteria, Bird / Avian (Other), Fish (Other), Mammal (Other), Plant
Nonreactive Species
Protozoa / Protozoan Parasite
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: IF/ICC
1:500 - 1:2000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Human Alpha Tubulin
Antigen Species
Human
Epitope
A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.61 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.