LSBio
IHC-plus™ Polyclonal Rabbit anti-Human Alpha Tubulin Antibody (aa425-451, IHC, IF, WB)

Anti-TUBA1 / Tubulin Alpha 1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human Alpha Tubulin Antibody (aa425-451, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Bovine, Chicken, Human, Mouse, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B722-50

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Overview

Description
Alpha Tubulin antibody LS-B722 is an unconjugated rabbit polyclonal antibody to Alpha Tubulin (aa425-451) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Bov, Ck, Hu, Ms, Rt
Predicted Reactivity
Bird / Avian (Other), Bacteria, Fish (Other), Mammal (Other), Other
Nonreactive Species
Other
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:90000
Recommended Dilution: IF/ICC
1:500 - 1:2000
Recommended Dilution: IHC-P
2.5 µg/ml
Recommended Dilution: WB
1:1000 - 1:5000

Target

Target
Alpha Tubulin
Antigen Species
Human
Specificity
A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
Epitope
A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.85 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.