LSBio
Polyclonal Rabbit anti-Chicken LYZ / Lysozyme Antibody (Lyophilized, HRP, IHC, WB)

Western Blot of Rabbit anti-Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: ~1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4 degrees C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4 degrees C. Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Rabbit anti-Chicken LYZ / Lysozyme Antibody (Lyophilized, HRP, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Chicken
Format:Lyophilized
See all Chicken LYZ / Lysozyme antibodies →
$564.00each
Catalog Number: LS-C209251-100

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Overview

Description
Lysozyme antibody LS-C209251 is an HRP-conjugated rabbit polyclonal antibody to chicken Lysozyme (LYZ). Validated for ELISA, IHC, IP and WB.
Application
ELISA, IHC, IP, WB
Reactivity
Ck
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
1,4-beta-N-acetylmuramidase C | LZM | Lysozyme | Lysozyme (renal amyloidosis) | LYZ | Renal amyloidosis | Lysozyme C | Gal d 4 | P00698
Recommended Dilution: ELISA
1:10000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:1000 - 1:5000
UniProt Number
P61626
SwissProt
P61626

Target

Target
Chicken LYZ / Lysozyme
Antigen Species
Chicken
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Lysozyme [Hen Egg White]. Cross reactivity against Lysozyme from other tissues and species may occur but have not been specifically determined.
Gene Name
LYZ

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Lysozyme Antibody was produced by repeated immunizations with hen egg white Lysozyme protein.

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Store vial at 4°C prior to opening. Restore with 0.1 ml of deionized water or equivalent. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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