Western Blot of rabbit anti-Lysozyme antibody. (Left Blot-grey) Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Biotin Conjugated Rabbit anti Lysozyme antibody. Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: ~1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:1,000 with Block: MB-070 overnight at 4°C. (Right Blot-red) Blot shown on right shows detection of 0.5 ug reduced (R) and nonreduced (NR) Lysozyme using p/n 200-401-072 lot 26517 (1:1000 in MB-070) with Dylight 549 conjugated secondary antibody. Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Predicted/Observed size: 4.9kDa, 5 kDa for Lysozyme. Other band(s): none.
LYZ / Lysozyme Antibody
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Chicken |
| Format: | Liquid |
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- Description
- Lysozyme antibody LS-C744954 is an unconjugated rabbit polyclonal antibody to chicken Lysozyme (LYZ). Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Ck
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 1,4-beta-N-acetylmuramidase C | LZM | Lysozyme | Lysozyme (renal amyloidosis) | LYZ | Renal amyloidosis | Lysozyme C | Gal d 4 | P00698
- Recommended Dilution: ELISA
- 1:25000
- Recommended Dilution: IHC
- 1:500 - 1:2000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P61626
- NCBI GenBank
- NM_000239 | NP_000230.1
- SwissProt
- P61626
Target
- Target
- Chicken LYZ / Lysozyme
- Antigen Species
- Chicken
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lysozyme (Hen Egg White). A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined.
- Gene Name
- LYZ
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl