POLR2A / RNA polymerase II Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Frozen, Western Blot |
| Reactivity: | Arthropod (Insect / Arachnid / Crustacean), Bovine, Dog, Fruit Fly, Hamster, Horse, Human, Mammal (Other), Mouse, Non-Human Primate, Rabbit, Rat, Xenopus, Zebrafish |
| Format: | Liquid |
$926.00each
Catalog Number: LS-C26239-100
Catalog Number: LS-C26239
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- RNA polymerase II antibody LS-C26239 is an unconjugated mouse monoclonal antibody to RNA polymerase II (POLR2A) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
- Application
- IHC, IHC-F, WB
- Reactivity
- Arth, Bov, Can, Dros, Ham, Hr, Hu, Mml, Ms, NHP, Rb, Rt, Xen, Zf
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- HRPB220 | HsRPB1 | POLR2 | RpIILS | RPOL2 | POLR2A | RNA Polymerase II | RPO2 | POLRA | RNA polymerase II subunit B1 | RPB1 | RPBh1
- UniProt Number
- P24928
- NCBI GenBank
- NM_000937 | NP_000928.1
- SwissProt
- P24928
Target
- Target
- Human POLR2A / RNA polymerase II
- Antigen Species
- Human
- Epitope
- Excellent for detection of transcription activity, e. g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200kD band of non-phosphorylated and 240kD band of phosphorylated Pol IIA. Reactivities on Cultured Cell Lines (tested so far): Drosophila chromosomes, PTK-2, HeLa, Hep2. Epitope recognized: 794DDYGPESRGFVENSYLA822. Species cross-reactivity: Human, insects.
- Gene Name
- POLR2A
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Isotype
- IgG1
Immunogen
- Immunogen
- RNA polymerase II. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.09% Sodium Azide
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.