Detection of Human and Mouse RNA Polymerase II by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug for WB), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNA Polymerase II antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
POLR2A / RNA polymerase II Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287522-2
Catalog Number: LS-C287522
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- Description
- RNA polymerase II antibody LS-C287522 is an unconjugated rabbit polyclonal antibody to RNA polymerase II (POLR2A) (C-Terminus) from human. It is reactive with human and mouse. Validated for IP and WB.
- Application
- IHC, IP, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HRPB220 | HsRPB1 | POLR2 | RpIILS | RPOL2 | POLR2A | RNA Polymerase II | RPO2 | POLRA | RNA polymerase II subunit B1 | RPB1 | RPBh1
- Recommended Dilution: WB
- 1:1000 - 1:10000
- UniProt Number
- P24928
- NCBI GenBank
- NM_000937 | NP_000928.1
- SwissProt
- P24928
Target
- Target
- Human POLR2A / RNA polymerase II
- Antigen Species
- Human
- Epitope
- The epitope recognized by A300-653A maps to the C-terminal repeat of human RNA Polymerase II (GeneID 5430).
- Gene Name
- POLR2A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 5430 corresponding to amino acids near the C-Terminus repeat of UniprotID: P24928
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate