Western Blot of rabbit anti-PIK3CB antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate. Lane 2: MDA-MB-435S WCL. Load: 35 µg per lane. Primary antibody: PIK3CB antibody at 1:1,000 for 3hrs at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 122 kDa for PIK3CB.
PIK3CB / PI3K Beta Antibody (N-Terminus)
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| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745404-100
Catalog Number: LS-C745404
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- Description
- PI3K Beta antibody LS-C745404 is an unconjugated rabbit polyclonal antibody to human PI3K Beta (PIK3CB) (N-Terminus). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- PI3-kinase p110 subunit beta | PI3K | PI3KCB | PI3K-beta | PIK3C1 | PIK3CB | PtdIns-3-kinase p110 | p110BETA | PI3-kinase subunit beta | PI3KBETA | PtdIns-3-kinase subunit beta
- Recommended Dilution: ELISA
- 1:50000
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- P42338
- NCBI GenBank
- NM_006219 | NP_006210.1
- SwissProt
- P42338
Target
- Target
- Human PIK3CB / PI3K Beta
- Antigen Species
- Human
- Epitope
- This antibody is specific for human PIK3C beta protein. A BLAST analysis was used to suggest cross-reactivity with PI3K-beta based on a 100% homology from human sources with the immunizing sequence. Expect partial cross reactivity to PIK3C beta from mouse and rat (93% homology) sources. Cross-reactivity with PIK3C beta from other sources has not been determined.
- Gene Name
- PIK3CB
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This affinity purified PIK3C-β antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-terminal region of human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.09 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl