LSBio
PHGDH Antibody

Immunofluorescence staining of PHGDH in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PHGDH polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.

PHGDH Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human PHGDH antibodies →
$212.00each
Catalog Number: LS-C816969-50

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Overview

Description
PHGDH antibody LS-C816969 is an unconjugated rabbit polyclonal antibody to human PHGDH. Validated for ICC, IF, IHC, IP and WB.
Application
ICC, IF, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
3-PGDH | 3PGDH | PGDH | Phosphoglycerate dehydrogenase | PHGDH | PDG | PGAD | PGDH3
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC-P
1:100 - 1:500
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
O43175
SwissProt
O43175

Target

Target
Human PHGDH
Antigen Species
Human
Epitope
Human PHGDH
Gene Name
PHGDH

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
E. coli-derived Human PHGDH fragment
Immunogen Type
Recombinant Protein Fragment

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.0, 0.03% Proclin 300
Volume
50 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.