LSBio
IHC-plus™ PHGDH Antibody (clone 4A3-1D6)

Anti-PHGDH antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ PHGDH Antibody (clone 4A3-1D6)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
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$383.00each
Catalog Number: LS-B5457-50

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Overview

Description
PHGDH antibody LS-B5457 is an unconjugated mouse monoclonal antibody to human PHGDH. Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IF, IHC, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
3-PGDH | 3PGDH | PGDH | Phosphoglycerate dehydrogenase | PHGDH | PDG | PGAD | PGDH3
Recommended Dilution: IF/ICC
1 - 10 µg/ml
Recommended Dilution: IHC-P
5 µg/ml
UniProt Number
O43175
SwissProt
O43175

Target

Target
PHGDH
Antigen Species
Human
Specificity
Human PHGDH
Epitope
Human PHGDH
Gene Name
PHGDH

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
4A3-1D6
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
PHGDH (AAH11262, 1 aa ~ 534 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4
Animal Free
No
Carrier-Free
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
High level PHGDH expression in breast is predominantly associated with keratin 5-positive cell lineage independently of malignancy. Gromova I, Gromov P, Honma N, Kumar S, Rimm D, Talman ML, Wielenga VT, Moreira JM. Molecular oncology. 2015;9:1636-54. PubMed: 26026368 PMC: PMC5528790

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