Detection of Human PCNA by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, rabbit anti-Actin antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 minutes. This image was taken for the unconjugated form of this product. Other forms have not been tested.

PCNA Antibody (aa225-C-term, Biotin)

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Antibody:	Rabbit Polyclonal
Applications:	Immunoprecipitation, Western Blot
Reactivity:	Human
Format:	Lyophilized

$482.00each

Catalog Number: LS-C287189-100

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Overview

Description: PCNA antibody LS-C287189 is a biotin-conjugated rabbit polyclonal antibody to human PCNA (aa225-C-term). Validated for IP and WB.
Application: IP, WB
Reactivity: Hu
Predicted Reactivity: Bovine, Dog, Guinea Pig, Mammal (Other), Non-Human Primate, Pig, Rabbit, Rat, Sheep
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: Cyclin | PCNA
Recommended Dilution: WB: 1:5000 - 1:20000
UniProt Number: P12004
NCBI GenBank: NM_002592 | NP_002583.1
SwissProt: P12004

Target

Target: Human PCNA
Antigen Species: Human
Epitope: Region between residue 225 and the C-terminus (residue 261) of human proliferating cell nuclear antigen using the numbering given in SwissProt entry P12004 (GeneID 5111).
Gene Name: PCNA

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: A portion of GeneID: 5111 corresponding to amino acids between 225 and the C-Terminus of UniprotID: P12004

Format

Conjugate/Label: Biotin
Format: Lyophilized
Formulation: Lyophilized from PBS, 20% Trehalose, 0.2% BSA, and 0.09% Sodium Azide
Volume: 100 Microliter
Preservative: Yes
Purification Method: Affinity Purified