SPP1 / Osteopontin Antibody
Catalog Number: LS-C136850-100
100 µL (1 mg/mL) / $339.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Anti-Osteopontin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
PathPlus™ SPP1 / Osteopontin Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Dog, Human, Mouse, Pig, Rat |
| Format: | Liquid |
$470.00each
Catalog Number: LS-B425-100
Catalog Number: LS-B425
For bulk orders or custom formulations:
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- Description
- Osteopontin antibody LS-B425 is an unconjugated rabbit polyclonal antibody to Osteopontin (SPP1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB. Cited in 2 publications.
- Application
- ELISA, IHC, IHC-P, IP, WB
- Reactivity
- Can, Hu, Ms, Por, Rt
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BSPI | BNSP | Bone sialoprotein 1 | ETA-1 | Nephropontin | OPN | Secreted phosphoprotein 1 | SPP-1 | SPP1/CALPHA1 fusion | Urinary stone protein | Uropontin | Osteopontin | Bone sialoprotein I | SPP1
- Recommended Dilution: ELISA
- 1:5000 - 1:20000
- Recommended Dilution: IHC-P
- 1:500
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P10451
- NCBI GenBank
- NM_000582 | NP_000573.1
- SwissProt
- P10451
Target
- Target
- SPP1 / Osteopontin
- Antigen Species
- Human
- Specificity
- The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.
- Epitope
- The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.
- Gene Name
- SPP1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, from the human osteopontin protein, conjugated to KLH using maleimide.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
- Volume
- 100 Microliter
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate, 0.15 M NaCl
Osteopontin induces beta-catenin signaling through activation of Akt in prostate cancer cells. Robertson BW, Chellaiah MA. Experimental cell research. 2010;316:1-11.
PubMed: 19850036 · PMC: PMC2787770
Effects of Cryopreservation on Canine Multipotent Stromal Cells from Subcutaneous and Infrapatellar Adipose Tissue. Duan W, Lopez MJ. Stem cell reviews. 2016;12:257-68.
PubMed: 26537238 · PMC: PMC4841859
