LSBio
IHC-plus™ SPP1 / Osteopontin Antibody (aa261-310)

Anti-SPP1 / Osteopontin antibody IHC staining of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ SPP1 / Osteopontin Antibody (aa261-310)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B9080-100

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Overview

Description
Osteopontin antibody LS-B9080 is an unconjugated rabbit polyclonal antibody to Osteopontin (SPP1) (aa261-310) from human. It is reactive with human, mouse and rat. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
BSPI | BNSP | Bone sialoprotein 1 | ETA-1 | Nephropontin | OPN | Secreted phosphoprotein 1 | SPP-1 | SPP1/CALPHA1 fusion | Urinary stone protein | Uropontin | Osteopontin | Bone sialoprotein I | SPP1
Recommended Dilution: IHC-P
1:200
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P10451
SwissProt
P10451

Target

Target
SPP1 / Osteopontin
Antigen Species
Human
Specificity
Osteopontin Antibody detects endogenous levels of total Osteopontin protein.
Epitope
Osteopontin Antibody detects endogenous levels of total Osteopontin protein.
Gene Name
SPP1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from human Osteopontin. (AA Range: 261-310)
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
100 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS, 150 mM NaCl

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