LSBio
PathPlus™ Polyclonal Rabbit anti-Human GPR49 / LGR5 Antibody (Cytoplasmic Domain, IHC, IF)

Human Colon: Formalin-Fixed Paraffin-Embedded (FFPE)

PathPlus™ Polyclonal Rabbit anti-Human GPR49 / LGR5 Antibody (Cytoplasmic Domain, IHC, IF)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general)
Reactivity:Human
Format:Liquid
See all GPR49 / LGR5 antibodies →
$449.00each
Catalog Number: LS-A1236-50

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Overview

Description
LGR5 antibody LS-A1236 is an unconjugated rabbit polyclonal antibody to human LGR5 (GPR49) (Cytoplasmic Domain). Validated for IF and IHC. Cited in 3 publications.
Application
IHC
Reactivity
Hu
Predicted Reactivity
Bovine, Hamster, Mammal (Other), Non-Human Primate, Rabbit
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
FEX | G-protein coupled receptor 49 | G protein-coupled receptor 49 | GPR67 | GRP49 | GPR49 | LGR5 | HG38 | G-protein coupled receptor 67
Recommended Dilution: IHC-P
2 - 3 µg/ml
UniProt Number
O75473
SwissProt
O75473

Target

Target
GPR49 / LGR5
Antigen Species
Human
Specificity
Human GPR49 / LGR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Epitope
Human GPR49 / LGR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Gene Name
LGR5

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Synthetic 20 amino acid peptide from 1st cytoplasmic domain of human GPR49 / LGR5. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%); Marmoset, Bovine, Bat (95%); Hamster, Elephant, Panda, Rabbit (90%); Mouse, Rat, Opossum (85%).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.1% Sodium Azide
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Expression of the adult intestinal stem cell marker Lgr5 in the metastatic cascade of colorectal cancer. Kleist B, Xu L, Li G, Kersten C. International journal of clinical and experimental pathology. 2011;4:327-35.

PubMed: 21577318 · PMC: PMC3093057


EP receptor expression in human intestinal epithelium and localization relative to the stem cell zone of the crypts. Olsen Hult LT, Kleiveland CR, Fosnes K, Jacobsen M, Lea T. PloS one. 2011;6:e26816.

PubMed: 22046368 · PMC: PMC3201980


Mesenchymal stem cells alleviate TNBS-induced colitis by modulating inflammatory and autoimmune responses. Chen QQ, Yan L, Wang CZ, Wang WH, Shi H, Su BB, Zeng QH, Du HT, Wan J. World journal of gastroenterology : WJG. 2013 Aug;19:4702-17.

PubMed: 23922467 · PMC: PMC3732842

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