Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
GPR49 / LGR5 Antibody (N-Terminus)
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general) |
| Reactivity: | Human, Mammal (Other), Non-Human Primate |
| Format: | Liquid |
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- Description
- LGR5 antibody LS-A1232 is an unconjugated rabbit polyclonal antibody to LGR5 (GPR49) (N-Terminus) from human. It is reactive with human, bat and monkey. Validated for ICC, IF and IHC. Cited in 3 publications.
- Application
- IHC
- Reactivity
- Hu, Mml, NHP
- Predicted Reactivity
- Bovine, Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- FEX | G-protein coupled receptor 49 | G protein-coupled receptor 49 | GPR67 | GRP49 | GPR49 | LGR5 | HG38 | G-protein coupled receptor 67
- Recommended Dilution: IHC-P
- 9 µg/ml
- UniProt Number
- O75473
- NCBI GenBank
- NM_003667 | NP_003658.1
- SwissProt
- O75473
Target
- Target
- GPR49 / LGR5
- Antigen Species
- Human
- Specificity
- N-terminus of human GPR49. BLAST analysis of the peptide immunogen showed no homology with other human proteins
- Epitope
- Human GPR49 / LGR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Gene Name
- LGR5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Synthetic 20 amino acid peptide from N-Terminus extracellular domain of human GPR49 / LGR5. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%); Elephant, Panda (95%); Mouse, Rat, Bovine (90%); Opossum (85%); Rabbit, Chicken (80%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.1% Sodium Azide
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
EP receptor expression in human intestinal epithelium and localization relative to the stem cell zone of the crypts. Olsen Hult LT, Kleiveland CR, Fosnes K, Jacobsen M, Lea T. PloS one. 2011;6:e26816.
PubMed: 22046368 · PMC: PMC3201980
Expression of LGR5, an intestinal stem cell marker, during each stage of colorectal tumorigenesis. Takeda K, Kinoshita I, Shimizu Y, Matsuno Y, Shichinohe T, Dosaka-Akita H. Anticancer research. 2011 Jan;31:263-70.
PubMed: 21273608
LGR5 is a marker of poor prognosis in glioblastoma and is required for survival of brain cancer stem-like cells. Nakata S, Campos B, Bageritz J, Bermejo JL, Becker N, Engel F, Acker T, Momma S, Herold-Mende C, Lichter P, Radlwimmer B, Goidts V. Brain pathology (Zurich, Switzerland). 2013 Jan;23:60-72.
PubMed: 22805276
Immunostaining of Lgr5, an Intestinal Stem Cell Marker, in Normal and Premalignant Human Gastrointestinal Tissue. Becker L, Huang Q, Mashimo H. TheScientificWorldJournal. 2008 November;8:1168-1176.
PubMed: 19030762 · PMC: PMC5848685
LGR5 expression predicts peritoneal recurrence after curative resection of primary colon cancer. Hiroshi Nagata, Soichiro Ishihara, Hiroyuki Abe, Tetsuo Ushiku, Junko Kishikawa, Toshiaki Tanaka, Keisuke Hata, Kazushige Kawai, Masashi Fukayama, Hiroaki Nozawa. British journal of cancer. 2019 May;120:996-1002.
PubMed: 31000786 · PMC: PMC6734652