OCLN / Occludin Antibody
Catalog Number: LS-C660344-100
100 µg / $338.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human
Format: Liquid
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PathPlus™ OCLN / Occludin Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Dog, Human, Mouse, Rat |
| Format: | Liquid |
$495.00each
Catalog Number: LS-B2187-50
Catalog Number: LS-B2187
For bulk orders or custom formulations:
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- Description
- Occludin antibody LS-B2187 is an unconjugated rabbit polyclonal antibody to Occludin (OCLN) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC, IP and WB. Cited in 4 publications.
- Application
- ELISA, IF, IHC, IHC-P, IP, WB
- Reactivity
- Can, Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Occludin | OCLN | BLCPMG
- Recommended Dilution: ELISA
- 0.1 - 1 µg/ml
- Recommended Dilution: IHC-P
- 2.5 µg/ml
- Recommended Dilution: WB
- 1 - 3 µg/ml
- UniProt Number
- Q16625
- NCBI GenBank
- NM_002538 | NP_002529.1
- SwissProt
- Q16625
Target
- Target
- OCLN / Occludin
- Antigen Species
- Human
- Specificity
- Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.
- Epitope
- Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.
- Gene Name
- OCLN
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- A GST fusion protein consisting of the C-Terminus 150 amino acids of human occludin fused to GST.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide
- Concentration
- 0.25 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
The tight-junction protein claudin-6 induces epithelial differentiation from mouse F9 and embryonic stem cells. Sugimoto K, Ichikawa-Tomikawa N, Satohisa S, Akashi Y, Kanai R, Saito T, Sawada N, Chiba H. PloS one. 2013;8:e75106.
PubMed: 24116027 · PMC: PMC3792957
ATF3 Protects Pulmonary Resident Cells from Acute and Ventilator-Induced Lung Injury by Preventing Nrf2 Degradation. Shan Y, Akram A, Amatullah H, Zhou DY, Gali PL, Maron-Gutierrez T, Gonz[Character e1]lez-L[Character f3]pez A, Zhou L, Rocco PR, Hwang D, Albaiceta GM, Haitsma JJ, Dos Santos CC. Antioxidants & redox signaling. 2014 Mar;22:651-68.
PubMed: 25401197 · PMC: PMC4346377
TLR3 deficiency exacerbates the loss of epithelial barrier function during genital tract Chlamydia muridarum infection. Kumar R, Gong H, Liu L, Ramos-Solis N, Seye CI, Derbigny WA. PloS one. 2019 January;14:e0207422.
PubMed: 30625140 · PMC: PMC6326510
Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland. Atsuko Y Higashi , Tomohito Higashi, Kyoko Furuse, Kana Ozeki, Mikio Furuse , Hideki Chiba. Scientific reports. 2021 Nov;11:21642.
PubMed: 34737342 · PMC: PMC8568902
