LSBio
IHC-plus™ Monoclonal Mouse anti-Human OCLN / Occludin Antibody (clone OC-3F10, C-Terminus, IHC, IF, WB)

Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ Monoclonal Mouse anti-Human OCLN / Occludin Antibody (clone OC-3F10, C-Terminus, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Dog, Human, Mouse, Rat
Format:Liquid
See all OCLN / Occludin antibodies →
$495.00each
Catalog Number: LS-B2320-50

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Overview

Description
Occludin antibody LS-B2320 is an unconjugated mouse monoclonal antibody to Occludin (OCLN) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Can, Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Occludin | OCLN | BLCPMG
Recommended Dilution: ELISA
0.1 - 1 µg/ml
Recommended Dilution: IF/ICC
2 - 3 µg/ml
Recommended Dilution: IHC-P
5 - 10 µg/ml
Recommended Dilution: WB
0.1 - 1 µg/ml
UniProt Number
Q16625
SwissProt
Q16625

Target

Target
OCLN / Occludin
Antigen Species
Human
Specificity
Recognizes mammalian Occludin Protein. Species cross-reactivity: human, mouse, rat and canine.
Epitope
Recognizes mammalian Occludin Protein. Species cross-reactivity: human, mouse, rat and canine.
Gene Name
OCLN

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
OC-3F10
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
GST fusion protein consisting of the C-Terminus (~150aa) of human occludin.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination With Cell Polarization During Ameloblast Differentiation. Otsu K, Ida-Yonemochi H, Fujiwara N, Harada H. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2016 November;31:1943-1954. PubMed: 27218883

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