Anti-HSPD1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

PathPlus™ HSPD1 / HSP60 Antibody (clone LK-2)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:	Bovine, Dog, Chicken, Guinea Pig, Hamster, Human, Mouse, Non-Human Primate, Plant, Pig, Rabbit, Rat, C. elegans
Format:	Liquid

$383.00each

Catalog Number: LS-B3678-50

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: HSP60 antibody LS-B3678 is an unconjugated mouse monoclonal antibody to HSP60 (HSPD1) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB.
Application: IHC, IHC-P, IP, WB
Reactivity: Bov, Can, Ck, GP, Ham, Hu, Ms, NHP, Plnt, Por, Rb, Rt, C.el
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: 60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSPD1 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
Recommended Dilution: IHC-P: 10 µg/ml
Recommended Dilution: IP: 10 µg/ml
Recommended Dilution: WB: 0.25 µg/ml
UniProt Number: P10809
NCBI GenBank: NM_002156 | NP_002147.2
SwissProt: P10809

Target

Target: HSPD1 / HSP60
Antigen Species: Human
Specificity: Detects an ~60 kD protein, corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, chicken, dog, guinea pig, hamster, monkey, pig, rabbit, plant (spinach), Borrelia, E. coli, helicobacter pylori, M. bovis (Hsp65), Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Yersinia entercolitica, and yeast origins. Deletion mutant analysis indicates that the epitope recognized by LK-2 is located between residues 383-419 of the human Hsp60 sequence (which corresponds to residues 356-393 of the mycobacterial Hsp60 sequence).
Epitope: Detects an ~60 kD protein, corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, chicken, dog, guinea pig, hamster, monkey, pig, rabbit, plant (spinach), Borrelia, E. coli, helicobacter pylori, M. bovis (Hsp65), Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Yersinia entercolitica, and yeast origins. Deletion mutant analysis indicates that the epitope recognized by LK-2 is located between residues 383-419 of the human Hsp60 sequence (which corresponds to residues 356-393 of the mycobacterial Hsp60 sequence).
Gene Name: HSPD1

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: LK-2
Isotype: IgG1
Origin Species: Human

Immunogen

Immunogen: Human HSP60 produced through recombinant DNA methods in E. coli.

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: Yes
Purification Method: Protein G Affinity

Storage & Handling

Storage Conditions: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Recommended Storage Buffer: PBS