LSBio
IHC-plus™ Monoclonal Mouse anti-Human HSPD1 / HSP60 Antibody (clone 4E101, aa383-419, IHC, WB)

Anti-HSPD1 / HSP60 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ Monoclonal Mouse anti-Human HSPD1 / HSP60 Antibody (clone 4E101, aa383-419, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Bacteria, Bovine, Chicken, Dog, Guinea Pig, Hamster, Human, Mouse, Non-Human Primate, Pig, Plant, Rabbit, Rat
Format:Liquid
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$567.00each
Catalog Number: LS-B10529-50

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Overview

Description
HSP60 antibody LS-B10529 is an unconjugated mouse monoclonal antibody to HSP60 (HSPD1) (aa383-419) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, FC, IHC, IHC-P, WB
Reactivity
Bac, Bov, Can, Ck, GP, Ham, Hu, Ms, NHP, Plnt, Por, Rb, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSPD1 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
Recommended Dilution: FC
10 µg/ml
Recommended Dilution: IHC-P
10 µg/ml
UniProt Number
P10809
SwissProt
P10809

Target

Target
HSPD1 / HSP60
Antigen Species
Human
Specificity
Recognizes human Hsp60 at ~60kD. Epitope: located within aa 383-419 of human Hsp60 (aa 356-393 of the mycobacterial Hsp60). Species cross-reactivity: Mouse, rat, bovine, C. elegans, chicken, canine, guinea pig, hamster, monkey, porcine, rabbit, plant (spinach, tomato), Borrelia, E. coli (GroEL), Helicobacter pylori, M. bovis (Hsp65), Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, white fly, Yersinia entercolitica and yeast.
Epitope
Recognizes human Hsp60 at ~60kD. Epitope: located within aa 383-419 of human Hsp60 (aa 356-393 of the mycobacterial Hsp60). Species Crossreactivity: mouse, rat, bovine, bacteria, C. elegans, chicken, canine, guinea pig, hamster, monkey, porcine, rabbit, plant.
Gene Name
HSPD1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
4E101
Origin Species
Human

Immunogen

Immunogen
Recombinant human Hsp60 produced in E. coli.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.