LSBio
PathPlus™ CDH1 / E Cadherin Antibody (C-Terminus)

Immunohistochemical analysis of E Cadherin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

PathPlus™ CDH1 / E Cadherin Antibody (C-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Bovine, Dog, Human, Non-Human Primate, Pig, Rabbit, Rat, Zebrafish
Format:Liquid
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$470.00each
Catalog Number: LS-B16416-50

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Overview

Description
E Cadherin antibody LS-B16416 is an unconjugated rabbit polyclonal antibody to E Cadherin (CDH1) (C-Terminus) from human. It is reactive with human, rat, bovine and other species. Validated for Flow, ICC, IF, IHC, IP and WB.
Application
FC, ICC, IF, IHC, IHC-P, IP, WB
Reactivity
Bov, Can, Hu, NHP, Por, Rb, Rt, Zf
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CDH1 | CD324 | CDHE | Cell-CAM 120/80 | E Cadherin | E-Cadherin | ECAD | Epithelial cadherin | Arc-1 | UVO | Cadherin-1 | CAM 120/80 | CD324 antigen | LCAM | Uvomorulin
Recommended Dilution: FC
1:100 - 1:200
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC-P
6 µg/ml
Recommended Dilution: IP
1:10 - 1:100
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P12830
SwissProt
P12830

Target

Target
CDH1 / E Cadherin
Antigen Species
Human
Specificity
Recognizes endogenous levels of E Cadherin protein.
Epitope
Recognizes endogenous levels of E Cadherin protein.
Gene Name
CDH1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human E Cadherin.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.