LSBio
IHC-plus™ Polyclonal Rabbit anti-Human CDH1 / E Cadherin Antibody (IHC, IF, WB)

Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

IHC-plus™ Polyclonal Rabbit anti-Human CDH1 / E Cadherin Antibody (IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all CDH1 / E Cadherin antibodies →
$383.00each
Catalog Number: LS-B16725-50

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Overview

Description
E Cadherin antibody LS-B16725 is an unconjugated rabbit polyclonal antibody to human E Cadherin (CDH1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CDH1 | CD324 | CDHE | Cell-CAM 120/80 | E Cadherin | E-Cadherin | ECAD | Epithelial cadherin | Arc-1 | UVO | Cadherin-1 | CAM 120/80 | CD324 antigen | LCAM | Uvomorulin
Recommended Dilution: IHC-P
10 µg/ml
UniProt Number
P12830
SwissProt
P12830

Target

Target
CDH1 / E Cadherin
Antigen Species
Human
Gene Name
CDH1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Recombinant Human Cadherin-1 protein (23-621AA)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Recommended Storage Buffer
0.01 M PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.