LSBio
PathPlus™ CD20 Antibody (clone rIGEL/773)

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

PathPlus™ CD20 Antibody (clone rIGEL/773)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Recombinant Monoclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin
Reactivity:Human
Format:Liquid
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$536.00each
Catalog Number: LS-B16225-100

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Overview

Description
CD20 antibody LS-B16225 is an unconjugated mouse recombinant monoclonal antibody to human CD20. Validated for Flow, IF and IHC.
Application
FC, IF, IHC, IHC-P
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Recombinant Antibodies
Alternative Names
B-lymphocyte antigen CD20 | B1 | Bp35 | CD20 | CD20 antigen | CD20 receptor | LEU-16 | CVID5 | MS4A1
Recommended Dilution: FC
1 - 2 µg/10E6 cells
Recommended Dilution: IF/ICC
1 - 2 µg/ml
Recommended Dilution: IHC-P
1:50
UniProt Number
P11836
NCBI GenBank
NM_021950 | NP_690605.1
SwissProt
P11836

Target

Target
CD20
Antigen Species
Human
Specificity
Recognizes a protein of 30-33 kDa , which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
Epitope
Recognizes a protein of 30-33 kDa , which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
Gene Name
MS4A1

Antibody

Host Species
Mouse
Clonality
Recombinant Monoclonal
Clone
rIGEL/773
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
Recombinant full-length human MS4A1 protein. Antigen Molecular Weight: 33-37 kDa
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Concentration
200 µg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Protein A/G Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 1 year.
Recommended Storage Buffer
10 mM PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.