Western Blot of rabbit Anti-Ffar4 antibody. Lane 1: Ladder (Opal Prestained) p/n MB-210-0500. Lane 2: Raw 264.7 WCL p/n W10-001-369. Lane 3: NIH/3T3 WCL p/n W10-000-358. Lane 4: PC-12 WCL p/n W12-001-GL9. Lane 5: Ladder (Opal Prestained) p/n MB-210-0500. Load: 35 µg per lane. Primary antibody: Ffar4 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Gt-a-Rb HRP secondary antibody 611-103-122 at 1:70,000 for 30 min at RT. Block: MB-073 BlockOut Universal Blocking Buffer for 30 min at RT. Predicted/Observed size: 40.8 kDa, observed ~48 kDa band due to glycosylation of Ffar4 protein.
O3FAR1 / GPR120 Antibody (N-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745417-25
Catalog Number: LS-C745417
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- Description
- GPR120 antibody LS-C745417 is an unconjugated rabbit polyclonal antibody to mouse GPR120 (O3FAR1) (N-Terminus). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Ms
- Predicted Reactivity
- Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- FFAR4 | Free fatty acid receptor 4 | GT01 | GPR129 | GPR120 | O3FAR1 | PGR4 | BMIQ10
- Recommended Dilution: ELISA
- 1:50000
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- Q5NUL3
- NCBI GenBank
- NM_181745 | NP_859529.2
- SwissProt
- Q5NUL3
Target
- Target
- Mouse O3FAR1 / GPR120
- Antigen Species
- Mouse
- Epitope
- A BLAST analysis was used to suggest reactivity with this protein in rat based on 100% homology match for the immunogen sequence.
- Gene Name
- FFAR4
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Affinity purified Anti-Ffar4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the N-Terminus portion of mouse Ffar4 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl