HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.
NONO / P54NRB Antibody (clone OTI3F8)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Dog, Human, Mouse, Non-Human Primate |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C173975-100
Catalog Number: LS-C173975
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- P54NRB antibody LS-C173975 is an unconjugated mouse monoclonal antibody to P54NRB (NONO) from human. It is reactive with human, mouse, dog and other species. Validated for IF, IHC and WB.
- Application
- IF, IHC, IHC-P, WB
- Reactivity
- Can, Hu, Ms, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 55 kDa nuclear protein | NRB54 | NMT55 | NonO protein | p54 | p54(nrb) | p54NRB | NONO
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC-P
- 1:150
- Recommended Dilution: WB
- 1:200 - 1:1000
- UniProt Number
- Q15233
- NCBI GenBank
- NM_007363 | NP_031389.3
- SwissProt
- Q15233
Target
- Target
- Human NONO / P54NRB
- Antigen Species
- Human
- Epitope
- Human NONO / P54NRB
- Gene Name
- NONO
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI3F8
- Isotype
- IgG1
Immunogen
- Immunogen
- Human recombinant protein fragment corresponding to amino acids 184-385 of human NONO(NP_031389) produced in E. coli.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS