Fluorescent confocal image of SY5Y cells stained with NGFR antibody SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated NGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
NGFR / CD271 / TNR16 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C164863-400
Catalog Number: LS-C164863
For bulk orders or custom formulations:
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- Description
- TNR16 antibody LS-C164863 is an unconjugated mouse monoclonal antibody to TNR16 (NGFR / CD271) from human. It is reactive with human and mouse. Validated for IF, IHC and WB.
- Application
- IF, IHC, IHC-P, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CD271 | CD271 antigen | gp80-LNGFR | Nerve growth factor receptor | p75 ICD | p75(NTR) | p75NTR | TNFR superfamily, member 16 | NGF receptor | NGFR | TNFRSF16 | p75-NTR
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC-P
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P08138
- NCBI GenBank
- NM_002507 | NP_002498.1
- SwissProt
- P08138
Target
- Target
- Human NGFR / CD271 / TNR16
- Antigen Species
- Human
- Epitope
- This NGFR antibody is generated from mouse immunized with NGFR recombinant protein.
- Gene Name
- NGFR
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Isotype
- IgG2b
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Full length recombinant human NGFR (P08138).
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS with 0.09% (w/v) sodium azide
- Concentration
- 0.38 mg/mL
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS