LSBio
Monoclonal Rat anti-Human RPA2 / RFA2 / RPA34 Antibody (IF, WB)

Monoclonal Rat anti-Human RPA2 / RFA2 / RPA34 Antibody (IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rat Monoclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Hamster, Human, Mouse, Non-Human Primate, Rat
Format:Liquid
See all Human RPA2 / RFA2 / RPA34 antibodies →
$717.00each
Catalog Number: LS-C38952-100

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Overview

Description
RPA34 antibody LS-C38952 is an unconjugated rat monoclonal antibody to RPA34 (RPA2 / RFA2) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IP and WB. Cited in 3 publications.
Application
ICC, IF, IHC, IP, WB
Reactivity
Ham, Hu, Ms, NHP, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
RF-A protein 2 | RPA2 | RP-A p34 | RPA34 | Replication protein A2, 32kDa | RP-A p32 | REPA2 | Replication factor A protein 2 | Replication protein A2 (32kD) | RPA32
Recommended Dilution: IF/ICC
1:200
Recommended Dilution: IP
1:50
Recommended Dilution: WB
1:1000
UniProt Number
P15927
SwissProt
P15927

Target

Target
Human RPA2 / RFA2 / RPA34
Antigen Species
Human
Epitope
Detects endogenous levels of total RPA32 protein. RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol alpha, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen. Species cross-reactivity: Hamster, human, monkey, mouse and rat.
Gene Name
RPA2

Antibody

Host Species
Rat
Clonality
Monoclonal
Isotype
IgG1

Immunogen

Immunogen
Immunizing rats with recombinant full-length human MBP-RPA32 protein.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100ug/ml BSA, <0.02% Sodium Azide, 50% glycerol
Volume
100 Microliter
Preservative
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer
10 mM HEPES|150 mM NaCl

DNA double-strand break repair pathway choice is directed by distinct MRE11 nuclease activities. Shibata A, Moiani D, Arvai AS, Perry J, Harding SM, Genois MM, Maity R, van Rossum-Fikkert S, Kertokalio A, Romoli F, Ismail A, Ismalaj E, Petricci E, Neale MJ, Bristow RG, Masson JY, Wyman C, Jeggo PA, Tainer JA. Molecular cell. Jan;53:7-18.

PubMed: 24316220 · PMC: PMC3909494


Pre-exposure to ionizing radiation stimulates DNA double strand break end resection, promoting the use of homologous recombination repair. Nakajima NI, Hagiwara Y, Oike T, Okayasu R, Murakami T, Nakano T, Shibata A. PloS one. 2015;10:e0122582.

PubMed: 25826455 · PMC: PMC4380452


Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan. Meisenberg C, Ashour ME, El-Shafie L, Liao C, Hodgson A, Pilborough A, Khurram SA, Downs JA, Ward SE, El-Khamisy SF. Nucleic acids research. 2017;45:1159-1176.

PubMed: 28180300 · PMC: PMC5388393

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