LSBio
Monoclonal Rat anti-Human RPA2 / RFA2 / RPA34 Antibody (IF, WB)

Monoclonal Rat anti-Human RPA2 / RFA2 / RPA34 Antibody (IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rat Monoclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Hamster, Human, Mouse, Non-Human Primate, Rat
Format:Liquid
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$717.00each
Catalog Number: LS-C38952-100

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Overview

Description
RPA34 antibody LS-C38952 is an unconjugated rat monoclonal antibody to RPA34 (RPA2 / RFA2) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IP and WB. Cited in 3 publications.
Application
ICC, IF, IHC, IP, WB
Reactivity
Ham, Hu, Ms, NHP, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
RF-A protein 2 | RPA2 | RP-A p34 | RPA34 | Replication protein A2, 32kDa | RP-A p32 | REPA2 | Replication factor A protein 2 | Replication protein A2 (32kD) | RPA32
Recommended Dilution: IF/ICC
1:200
Recommended Dilution: IP
1:50
Recommended Dilution: WB
1:1000
UniProt Number
P15927
SwissProt
P15927

Target

Target
Human RPA2 / RFA2 / RPA34
Antigen Species
Hu
Epitope
Detects endogenous levels of total RPA32 protein. RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol alpha, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen. Species cross-reactivity: Hamster, human, monkey, mouse and rat.
Gene Name
RPA2

Antibody

Host Species
Rt
Clonality
Monoclonal
Isotype
IgG1

Immunogen

Immunogen
Immunizing rats with recombinant full-length human MBP-RPA32 protein.
Immunogen Type
Recom

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100ug/ml BSA, <0.02% Sodium Azide, 50% glycerol
Volume
100 Microliter
Preservative
Yes
Purification Method
Crude

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer
10 mM HEPES|150 mM NaCl
DNA double-strand break repair pathway choice is directed by distinct MRE11 nuclease activities. Shibata A, Moiani D, Arvai AS, Perry J, Harding SM, Genois MM, Maity R, van Rossum-Fikkert S, Kertokalio A, Romoli F, Ismail A, Ismalaj E, Petricci E, Neale MJ, Bristow RG, Masson JY, Wyman C, Jeggo PA, Tainer JA. Molecular cell. Jan;53:7-18. PubMed: 24316220 PMC: PMC3909494

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