Immunoprecipitation(IP) of RPA2 by using monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-RPA2 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
RPA2 / RFA2 / RPA34 Antibody (clone OTI4C3)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C114844-100
Catalog Number: LS-C114844
For bulk orders or custom formulations:
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- Description
- RPA34 antibody LS-C114844 is an unconjugated mouse monoclonal antibody to human RPA34 (RPA2 / RFA2). Validated for IF, IP and WB.
- Application
- IF, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- RF-A protein 2 | RPA2 | RP-A p34 | RPA34 | Replication protein A2, 32kDa | RP-A p32 | REPA2 | Replication factor A protein 2 | Replication protein A2 (32kD) | RPA32
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- P15927
- NCBI GenBank
- NM_002946 | NP_002937.1
- SwissProt
- P15927
Target
- Target
- Human RPA2 / RFA2 / RPA34
- Antigen Species
- Human
- Epitope
- Human RPA2 / RPA34
- Gene Name
- RPA2
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI4C3
- Isotype
- IgG1
Immunogen
- Immunogen
- Full length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 0.79 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS