LSBio
Monoclonal Mouse anti-Synthetic His Tag Antibody (clone AD1.1.10, HRP, WB)

Monoclonal Mouse anti-Synthetic His Tag Antibody (clone AD1.1.10, HRP, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Other
Format:Liquid
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$430.00each
Catalog Number: LS-C188123-0.1

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Overview

Description
His Tag antibody LS-C188123 is an HRP-conjugated mouse monoclonal antibody to synthetic His Tag. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Other
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:20 - 1:1000
Recommended Dilution: WB
1:1000 - 1:5000

Target

Target
Synthetic His Tag
Antigen Species
Other
Epitope
Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian ; ; and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
AD1.1.10
Isotype
IgG1

Immunogen

Immunogen
PAX6 transcription factor linked to histidine tag.

Format

Conjugate/Label
Horseradish Peroxidase
Format
Liquid
Formulation
PBS, 0.01% Thimerosal
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze. Store undiluted. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.