LSBio
His Tag Antibody (clone AD1.1.10, FITC)

Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualized with FITC conjugated Mouse anti-HIS tag antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

His Tag Antibody (clone AD1.1.10, FITC)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunofluorescence
Reactivity:Other
Format:Liquid
See all Synthetic His Tag antibodies →
$352.00each
Catalog Number: LS-C57341-0.1

For bulk orders or custom formulations:

Request a Quote

Overview

Description
His Tag antibody LS-C57341 is an FITC-conjugated mouse monoclonal antibody to synthetic His Tag. Validated for Flow and IF. Cited in 3 publications.
Application
FC, IF
Reactivity
Other
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: FC
1:10

Target

Target
Synthetic His Tag
Antigen Species
Other
Epitope
Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian ; ; and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
AD1.1.10
Isotype
IgG1

Immunogen

Immunogen
PAX6 transcription factor linked to histidine tag.

Format

Conjugate/Label
Fluorescein Isothiocyanate
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide, 1% BSA
Concentration
0.1 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

The Staphylococcus aureus superantigen SElX is a bifunctional toxin that inhibits neutrophil function. Tuffs SW, James DBA, Bestebroer J, Richards AC, Goncheva MI, O'Shea M, Wee BA, Seo KS, Schlievert PM, Lengeling A, van Strijp JA, Torres VJ, Fitzgerald JR. PLoS pathogens. 2017;13:e1006461.

PubMed: 28880920 · PMC: PMC5589267

Novel FGFR4-Targeting Single-Domain Antibodies for Multiple Targeted Therapies against Rhabdomyosarcoma. Nagjie Alijaj, Sandrine Moutel, Zelia L Gouveia, Maxim Gray, Maurizio Roveri, Dzhangar Dzhumashev, Florian Weber, Gianmarco Meier, Paola Luciani, Jochen K Rössler, Beat W Schäfer, Franck Perez, Michele Bernasconi. Cancers. 2020 November;12.

PubMed: 33182650 · PMC: PMC7696840

Minimalistic mycoplasmas harbor different functional toxin-antitoxin systems. Virginia Hill , Hatice Akarsu, Rubén Sánchez Barbarroja, Valentina L Cippà, Peter Kuhnert, Martin Heller, Laurent Falquet, Manfred Heller, Michael H Stoffel, Fabien Labroussaa, Joerg Jores. PLoS genetics. 2021 Oct;17:e1009365.

PubMed: 34673769 · PMC: PMC8562856

Submit a Publication

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.