LSBio
Monoclonal Mouse anti-Human HRAS / H-Ras Antibody (clone OTI1C8, IHC, IF, WB)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.

Monoclonal Mouse anti-Human HRAS / H-Ras Antibody (clone OTI1C8, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human HRAS / H-Ras antibodies →
$443.00each
Catalog Number: LS-C340614-100

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Overview

Description
H-Ras antibody LS-C340614 is an unconjugated mouse monoclonal antibody to human H-Ras (HRAS). Validated for IF, IHC and WB. Cited in 1 publication.
Application
IF, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
C-H-RAS | C-has/bas p21 protein | C-ras-Ki-2 activated oncogene | C-HA-RAS1 | CTLO | H-ras | H-RASIDX | GTP- and GDP-binding peptide B | GTPase HRas | H-Ras-1 | HAMSV | HRAS | HRAS1 | K-RAS | Ha-Ras | Ha-Ras1 proto-oncoprotein | Transforming protein p21 | N-ras | p21ras | RASH1 | C-BAS/HAS | C-ha-ras | p19 H-RasIDX protein
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: IHC-P
1:150
Recommended Dilution: WB
1:2000
UniProt Number
P01112
SwissProt
P01112

Target

Target
Human HRAS / H-Ras
Antigen Species
Human
Epitope
Human HRAS / H-Ras
Gene Name
HRAS

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
OTI1C8
Isotype
IgG1

Immunogen

Immunogen
Full length human recombinant protein of human HRAS(NP_005334) produced in HEK293T cell.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
DNA methylation-regulated QPCT promotes sunitinib resistance by increasing HRAS stability in renal cell carcinoma. Zhao T, Bao Y, Gan X, Wang J, Chen Q, Dai Z, Liu B, Wang A, Sun S, Yang F, Wang L. Theranostics. 2019 August;9:6175-6190. PubMed: 31534544 PMC: PMC6735520

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