LSBio
Monoclonal Mouse anti-Human FCGR2 / CD32 Antibody (clone AT10, Low Endotoxin, IHC)

Monoclonal Mouse anti-Human FCGR2 / CD32 Antibody (clone AT10, Low Endotoxin, IHC)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunoprecipitation
Reactivity:Human
Format:Liquid
See all Human FCGR2 / CD32 antibodies →
$654.00each
Catalog Number: LS-C187457-0.5

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Overview

Description
CD32 antibody LS-C187457 is an unconjugated mouse monoclonal antibody to human CD32 (FCGR2). Validated for Flow, IHC and IP. Cited in 1 publication.
Application
FC, IHC, IHC-F, IP
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: IHC-F
1:500 - 1:1000

Target

Target
Human FCGR2 / CD32
Antigen Species
Human
Epitope
Recognizes the human CD32 antigen, a ~40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Clone AT10 blocks the binding of IgG to Fc gamma RII.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
AT10
Isotype
IgG1

Immunogen

Immunogen
K562 cell line.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS
Concentration
1 mg/mL
Preservative
No
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS
Factor H-IgG Chimeric Proteins as a Therapeutic Approach against the Gram-Positive Bacterial Pathogen Streptococcus pyogenes. Blom AM, Magda M, Kohl L, Shaughnessy J, Lambris JD, Ram S, Ermert D. Journal of immunology (Baltimore, Md. : 1950). 2017 December;199:3828-3839. PubMed: 29084837 PMC: PMC5698128

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