HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.
FCGR2 / CD32 Antibody (clone OTI19C10)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C114742-100
Catalog Number: LS-C114742
For bulk orders or custom formulations:
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- Description
- CD32 antibody LS-C114742 is an unconjugated mouse monoclonal antibody to human CD32 (FCGR2). Validated for Flow, IF, IHC, IP and WB.
- Application
- FC, IF, IHC, IHC-P, IP, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: FC
- 1:100
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC-P
- 1:50
- Recommended Dilution: WB
- 1:2000
Target
- Target
- Human FCGR2 / CD32
- Antigen Species
- Human
- Epitope
- Reacts with human CD32A, not yet tested for cross reactivity with CD32B.
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI19C10
- Isotype
- IgG1
Immunogen
- Immunogen
- Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS