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Monoclonal Mouse anti-Human F8 / FVIII / Factor VIII Antibody (clone RFF-VIIIC/8, WB)

Recombinant Factor VIII detected with Mouse anti-Human Factor VIII

Monoclonal Mouse anti-Human F8 / FVIII / Factor VIII Antibody (clone RFF-VIIIC/8, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Radioimmunoassay, Western Blot
Reactivity:Human
Format:Liquid
See all Human F8 / FVIII / Factor VIII antibodies →
$519.00each
Catalog Number: LS-C188516-0.5

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Overview

Description
Factor VIII antibody LS-C188516 is an unconjugated mouse monoclonal antibody to human Factor VIII (F8 / FVIII). Validated for ELISA, RIA and WB.
Application
ELISA, IHC, RIA, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
AHF | Antihemophilic factor | Coagulation factor VIIIc | Coagulation factor VIII | DXS1253E | Factor VIII F8B | FVIII | HEMA | F8 | F8B | Factor VIII | Procoagulant component | F8C
Recommended Dilution: ELISA
1:500 - 1:15000
UniProt Number
P00451
NCBI GenBank
NM_000132 | NP_000123.1
SwissProt
P00451

Target

Target
Human F8 / FVIII / Factor VIII
Antigen Species
Human
Epitope
Recognizes human Factor VIII, an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause hemophilia A, a disorder characterized by the body's inability to control blood clotting. This could result in severe blood loss, even with minor injuries. Clone RFF-VIIIC/8 is a very potent coagulation inhibitor. It recognizes an epitope towards the N-terminus of full-length Factor VIII. It also recognizes the 210kD, 90kD and 40kD cleavage products. This clone does not cross-react with von Willebrand factor.
Gene Name
F8

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
RFF-VIIIC/8
Isotype
IgG1

Immunogen

Immunogen
Affinity purified human Factor VIII.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.