Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, AF647)
Catalog Number: LS-C810655
2 sizes / from $259.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse
Format: Liquid
Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4 degrees C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none. This image was taken for the unmodified form of this product. Other forms have not been tested.
Monoclonal Mouse anti-Human AKT1 Antibody (clone 14E5.A2.B2.H9, Internal, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$2652.00each
Catalog Number: LS-C209206-1
Catalog Number: LS-C209206
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- Description
- AKT1 antibody LS-C209206 is an unconjugated mouse monoclonal antibody to human AKT1 (Internal). Validated for ELISA, Flow, IHC and WB.
- Application
- ELISA, FC, IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- AKT | AKT1 | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P31749
- NCBI GenBank
- NM_005163 | NP_005154.2
- SwissProt
- P31749
Target
- Target
- Human AKT1
- Antigen Species
- Human
- Epitope
- The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
- Gene Name
- AKT1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 14E5.A2.B2.H9
- Isotype
- IgG2a
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Anti-AKT1 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
- Concentration
- 1 mg/mL
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
