LSBio
IHC-plus™ AKT1 Antibody (phospho-Thr308)

Anti-AKT1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ AKT1 Antibody (phospho-Thr308)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
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$516.00each
Catalog Number: LS-B1190-50

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Overview

Description
AKT1 antibody LS-B1190 is an unconjugated rabbit polyclonal antibody to AKT1 (pThr308) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, FC, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
AKT | AKT1 | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Recommended Dilution: ELISA
1:15000
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1:1000
UniProt Number
P31749
SwissProt
P31749

Target

Target
AKT1
Antigen Species
Human
Specificity
Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested.
Epitope
Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested.
Gene Name
AKT1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Anti-AKT pT308 polyclonal antibody was produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to residues surrounding threonine 308 of human AKT1 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.97 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.